Prüfer D, Schmitz J, Tacke E, Kull B, Rohde W
Max-Planck-Institut für Züchtungsforschung, Köln, Germany.
Mol Gen Genet. 1997 Feb 20;253(5):609-14. doi: 10.1007/s004380050363.
A full-length cDNA copy (PLRVfl) of potato leafroll virus (PLRV) was constructed and examined in vivo for its biological activities by transient expression experiments with plasmid DNA or in vitro transcribed RNA. In addition, PLRVfl cDNA was stably introduced into the genome of potato plants by Agrobacterium-mediated leaf disc transformation. Both transient and stable expression of PLRVfl resulted in the synthesis of genomic and subgenomic PLRV RNAs. Transgenic plants accumulated the 17-kDa movement protein and displayed the typical symptoms of PLRV infection. This is the first example of the constitutive expression of a phloem-limited virus in planta.
构建了马铃薯卷叶病毒（PLRV）的全长cDNA拷贝（PLRVfl），并通过质粒DNA瞬时表达实验或体外转录RNA在体内检测其生物学活性。此外，通过农杆菌介导的叶盘转化将PLRVfl cDNA稳定导入马铃薯植株基因组。PLRVfl的瞬时表达和稳定表达均导致基因组和亚基因组PLRV RNA的合成。转基因植物积累了17 kDa运动蛋白，并表现出PLRV感染的典型症状。这是韧皮部限制型病毒在植物中组成型表达的首个实例。
