Sadowy E, Pluta K, Gronenborn B, Hulanicka D
Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw.
Acta Biochim Pol. 1998;45(2):611-9.
Infectious transcripts play a key role in the research on plant viruses at the molecular level. A number of cDNA clones covering the whole genome of the Polish isolate of potato leafroll virus were constructed. Four overlapping clones were selected and assembled using restriction sites. The full copy was positioned between T7 RNA polymerase promoter and unique ScaI site. The full-length capped transcripts of the sequence of the viral genome synthesised in vitro were able to replicate in protoplasts and to produce the viral coat protein.
感染性转录本在植物病毒分子水平研究中起着关键作用。构建了一系列覆盖波兰马铃薯卷叶病毒分离株全基因组的cDNA克隆。选择了四个重叠克隆，并利用限制性酶切位点进行组装。完整拷贝位于T7 RNA聚合酶启动子和独特的ScaI位点之间。体外合成的病毒基因组序列的全长加帽转录本能够在原生质体中复制并产生病毒外壳蛋白。
