乳铁蛋白和转铁蛋白对8-苯胺基萘-1-磺酸盐的铁依赖性结合。
Iron-dependent binding of 8-anilinonaphthalene-1-sulphonate by both lactoferrin and transferrin.
作者信息
Bilić N, Casey M, Blanc B
出版信息
Biochem J. 1976 Jul 1;157(1):233-5. doi: 10.1042/bj1570233.
Abstract
Fluorescence of 8-anilinonaphthalene-1-sulphonate is enhanced by both lactoferrin and transferrin. The enhancement is relatively low between pH 4 and 8, and high at below pH 4. At physiological pH the fluorescence enhancement is higher with the iron-deprived than with the iron-saturated proteins. Binding of iron by lactoferrin is associated with lowering affinity for the dye.
摘要
乳铁蛋白和转铁蛋白均可增强1-萘胺-8-磺酸的荧光。在pH 4至8之间荧光增强相对较低，而在pH 4以下则较高。在生理pH条件下，缺铁的蛋白质比铁饱和的蛋白质荧光增强更高。乳铁蛋白结合铁与降低对染料的亲和力有关。
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