Ferroni P, Lenti L, Martini F, Ciatti F, Pontieri G M, Gazzaniga P P
Department of Experimental Medicine and Pathology, University of Rome La Sapienza, Italy.
Thromb Haemost. 1997 Mar;77(3):548-54.
Gangliosides may play functional roles in platelet physiology, therefore this study has been designed to evaluate whether changes in ganglioside composition may occur as a consequence of platelet activation. The results obtained indicate that lactosylceramide and GM3 are the major glycosphingolipids of human platelets. The lipid-bound sialic acid (LBSA) content was 1.27 +/- 0.04 micrograms/mg of protein. Resting platelets did not express GD3; GD3 was synthesized upon platelet activation (24 +/- 8 ng/mg of protein). The stimulation of platelets with adenosine diphosphate showed the appearance of GD3 even in the absence of degranulation. Finally, incorporation of pyrene-labeled GM3 into platelet membranes, followed by stimulation with adenosine diphosphate, resulted in the appearance of a fluorescent band comigrating with GD3. The present studies indicate that sialytransferase activation may occur as an early event following platelet stimulation, leading to GD3 synthesis mainly from the GM3 pool.
神经节苷脂可能在血小板生理过程中发挥功能性作用，因此本研究旨在评估神经节苷脂组成的变化是否可能作为血小板激活的结果而发生。所获得的结果表明，乳糖基神经酰胺和GM3是人类血小板的主要糖鞘脂。脂质结合唾液酸（LBSA）含量为1.27±0.04微克/毫克蛋白质。静息血小板不表达GD3；GD3在血小板激活时合成（24±8纳克/毫克蛋白质）。用二磷酸腺苷刺激血小板，即使在没有脱颗粒的情况下也显示出GD3的出现。最后，将芘标记的GM3掺入血小板膜中，然后用二磷酸腺苷刺激，导致出现与GD3共迁移的荧光带。目前的研究表明，唾液酸转移酶激活可能作为血小板刺激后的早期事件发生，主要导致从GM3池合成GD3。
