[利用高效薄层色谱法（HPTLC）检测人乳中的神经节苷脂]

[Detection of gangliosides in human milk with a high performance thin layer chromatography (HPTLC)].

作者信息

Pan X, Hua T, Wu Y

机构信息

Department of Basic Pediatrics, Second Clinical College, China Medical University, Shenyang 110003, China.

出版信息

Zhonghua Yu Fang Yi Xue Za Zhi. 2001 Mar;35(2):111-3.

PMID:11413682

Abstract

OBJECTIVES

To determine the ganglioside composition in human milk during lactation, and to evaluate the biological significance.

METHODS

The gangliosides extracted from 68 milk samples were examined with a high performance thin layer chromatography (HPTLC).

RESULTS

The concentration of total lipid bound sialic acid was 7.8-10.4 microliters/ml. Four gangliosides were found and designated as GM3, GD3, GX1 and GX2, respectively. GX1 and GX2, which had not been described previously, were tentatively assumed to be gangliosides of the c-pathway because they did not react to the GA1 antibody after sialidase treatment. GD3 and GM3 were the major gangliosides in human milk (accounted for 50%-65%). CM3 decreased sharply at eight days postpartum. Both GM3 and GD3 changed significantly during lactation (GM3, r = 0.65, P < 0.01; GD3, r = -0.58, P < 0.01).

CONCLUSIONS

Four gangliosides, GM3, GD3, GX1 and GX2 were found in human milk. Both GM3 and GD3 changed significantly during lactation.

摘要

目的

测定哺乳期人乳中的神经节苷脂组成，并评估其生物学意义。

方法

采用高效薄层色谱法（HPTLC）检测从68份乳汁样本中提取的神经节苷脂。

结果

总脂质结合唾液酸浓度为7.8 - 10.4微升/毫升。发现了四种神经节苷脂，分别命名为GM3、GD3、GX1和GX2。GX1和GX2此前未被描述，由于经唾液酸酶处理后它们不与GA1抗体反应，暂被认为是c途径的神经节苷脂。GD3和GM3是人乳中的主要神经节苷脂（占50% - 65%）。产后8天CM3急剧下降。GM3和GD3在哺乳期均有显著变化（GM3，r = 0.65，P < 0.01；GD3，r = -0.58，P < 0.01）。