Thiesen B, Greenwood B, Brieske N, Achtman M
Max-Planck Institut für molekulare Genetik, Berlin, Germany.
Vaccine. 1997 Feb;15(2):209-19. doi: 10.1016/s0264-410x(96)00138-7.
Sera were taken over a 5 year period from Gambian children vaccinated in 1983, when aged 1-4 years, with A + C meningococcal capsular polysaccharide, ELISA tests were devised to determine the concentrations of immunoglobulin A, G and M reacting with A polysaccharide and of IgG reacting with Opc protein, IgA1 protease and an internal 104 mer peptide derived from IgA1 protease. Vaccination resulted in a brief rise of antibodies to A polysaccharide followed by decline to pre-immunization levels. IgM levels were very high even before vaccination. Antibodies to Opc protein stimulated by natural exposure also declined over the 5 year period. In contrast, antibodies stimulated by natural exposure to IgA1 protease or to the internal peptide remained constant or increased (final geometric mean level of 47 micrograms IgG ml-1). We speculate that healthy carriage of Neisseria meningitidis or Haemophilus influenzae is responsible for this increase in IgG concentration.
血清取自1983年接种A + C脑膜炎球菌荚膜多糖疫苗的冈比亚儿童，当时他们年龄在1至4岁之间。设计了酶联免疫吸附测定（ELISA）试验来确定与A多糖反应的免疫球蛋白A、G和M以及与Opc蛋白、IgA1蛋白酶和源自IgA1蛋白酶的内部104聚体肽反应的IgG的浓度。接种疫苗导致抗A多糖抗体短暂上升，随后降至免疫前水平。即使在接种疫苗之前，IgM水平也非常高。在5年期间，自然暴露刺激产生的抗Opc蛋白抗体也有所下降。相比之下，自然暴露于IgA1蛋白酶或内部肽刺激产生的抗体保持不变或增加（最终几何平均水平为47微克IgG/毫升）。我们推测，脑膜炎奈瑟菌或流感嗜血杆菌的健康携带是导致IgG浓度增加的原因。
