Reinhart W H, Schlappritzi E, Baerlocher G M
Kantonsspital, Chur, Switzerland.
Acta Haematol. 1997;97(3):132-6. doi: 10.1159/000203670.
The influence of echinocytosis induced by ATP depletion on erythrophagocytosis was analysed. Monocytes were isolated from peripheral blood of healthy, rhesus-positive volunteers and cultured for a total time of 6 h at 37 degrees C with 5% CO2. Erythrocytes from the same donor, either fresh (discocytes) or incubated at 37 degrees C for 48 h (spheroechinocytes) were added. Phagocytosis was quantified by measuring the chemiluminescence of the ingested heme. The rate of phagocytosis was 0.03 +/- 0.15 discocytes per phagocytic cell and 0.01 +/- 0.08 spheroechinocytes per phagocytic cell. Autologous serum did not affect this rate. The addition of anti-RhD immunoglobulins, however, increased phagocytosis drastically for discocytes and spheroechinocytes (4.22 +/- 2.09 and 5.24 +/- 2.58 erythrocytes per phagocytic cell, respectively). It is concluded that spheroechinocytosis induced by metabolic depletion, certainly one of the most severe forms of erythrocyte shape abnormalities, does not stimulate monocytes to remove these cells.
分析了由ATP耗竭诱导的棘红细胞增多症对红细胞吞噬作用的影响。从健康的恒河猴阳性志愿者外周血中分离出单核细胞，并在37℃、5%二氧化碳条件下共培养6小时。加入来自同一供体的新鲜红细胞（盘状红细胞）或在37℃孵育48小时的红细胞（球形棘红细胞）。通过测量摄入血红素的化学发光来定量吞噬作用。吞噬率为每个吞噬细胞0.03±0.15个盘状红细胞和每个吞噬细胞0.01±0.08个球形棘红细胞。自体血清不影响该比率。然而，添加抗RhD免疫球蛋白后，盘状红细胞和球形棘红细胞的吞噬作用急剧增加（分别为每个吞噬细胞4.22±2.09个和5.24±2.58个红细胞）。得出的结论是，代谢耗竭诱导的球形棘红细胞增多症，无疑是红细胞形状异常最严重的形式之一，并不会刺激单核细胞清除这些细胞。
