Sheremet'ev Yu A, Popovicheva A N, Rogozin M M, Levin G Ya
Tsitologiia. 2016;58(1):30-4.
An aggregation of erythrocytes in autologous plasma after blood storage for 14 days at 4 °C was studied using photometry and light microscopy. The decrease of ATP content, the formation of echinocytes and spheroechinocytes, the decrease of rouleaux form of erythrocyte aggregation were observed during the storage. On the other hand the aggregates of echinocytes were formed in the stored blood. The addition of plasma from the fresh blood didn't restore the normal discocytic shape and aggregation of erythrocytes in the stored blood. The possible mechanisms of erythrocytes and echinocytes aggregation are discussed.
采用光度测定法和光学显微镜研究了血液在4℃储存14天后自体血浆中红细胞的聚集情况。储存期间观察到ATP含量降低、棘形红细胞和球形棘形红细胞形成、红细胞聚集的缗钱状形态减少。另一方面,储存血液中形成了棘形红细胞聚集体。添加新鲜血液的血浆并不能恢复储存血液中红细胞的正常圆盘状形态和聚集。讨论了红细胞和棘形红细胞聚集的可能机制。