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PCR-based method for isolation of full-length clones and splice variants from cDNA libraries.

作者信息

Alphey L

机构信息

University of Manchester, School of Biological Sciences, England, UK.

出版信息

Biotechniques. 1997 Mar;22(3):481-4, 486. doi: 10.2144/97223st02.

DOI:10.2144/97223st02
PMID:9067026
Abstract

Most cDNA library screening procedures do not distinguish between full-length and incomplete clones and therefore may yield incomplete cDNA fragments. Thus, there is a widespread need for a method allowing the efficient selection of full-length clones. I present a rapid, PCR-based method that allows the simultaneous screening of > 10(6) cDNAs. The longest cDNA is identified in the first step so that incomplete clones may be eliminated from study at this stage to save time. The method also facilitates the identification and isolation of rare splice variants from a background of a more abundant variant.

摘要

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