Schmidt G, Gross J, Necas E, Neuwirt J
Acta Biol Med Ger. 1977;36(2):205-11.
In the present study the adequacy of erythrocytic criteria for the diagnosis of hypoxia has been investigated in animal experiments. Guinea pigs were exposed to a defined hypoxia (pO2 equals 48 mm Hg approximately equal to 9000 m height) for 24 and 72 hrs. With increasing duration of hypoxia there is observable a distinct rise in reticulocyte count, 59Fe incorporation and the proportion of erythroblasts in the bone marrow. Hematocrit and hemoglobin concentration have increased significantly after hypoxia compared with the control animals. The mean cellular hemoglobin content shows a marked fall with the duration of hypoxia. The density distribution curves show under hypoxia a shift to lower densities and, therefore, appear to be well suited for the diagnosis of hypoxic states. The activities of the erythrocytic enzymes, G-6-PD and GOT, are increased after 24 and 72 hrs of hypoxia. The GOT isoenzyme pattern is shifted with continued hypoxia in favour of the mitochondrial GOT. The changes of enzyme activities are not pronounced enough under hypoxic conditions to be used for diagnositc purposes.
在本研究中，已在动物实验中对用于诊断缺氧的红细胞标准的充分性进行了研究。将豚鼠暴露于特定的缺氧环境（pO2等于48 mmHg，约等于海拔9000米的高度）24小时和72小时。随着缺氧持续时间的增加，可观察到网织红细胞计数、59Fe掺入量以及骨髓中早幼红细胞比例明显升高。与对照动物相比，缺氧后血细胞比容和血红蛋白浓度显著增加。平均细胞血红蛋白含量随缺氧持续时间显著下降。密度分布曲线显示在缺氧状态下向较低密度偏移，因此，似乎非常适合用于诊断缺氧状态。缺氧24小时和72小时后，红细胞酶G-6-PD和GOT的活性增加。随着缺氧持续，GOT同工酶模式向有利于线粒体GOT的方向转变。在缺氧条件下，酶活性的变化不够明显，无法用于诊断目的。
