实验动物中红细胞衰老与酶活性之间的关系。

The relationship between red cell aging and enzyme activities in experimental animals.

Shimizu Y, Suzuki M

Department of Veterinary Medicine, Faculty of Agriculture, Gifu University, Japan.

Comp Biochem Physiol B. 1991;99(2):313-6. doi: 10.1016/0305-0491(91)90047-h.

Abstract

The relationship between red cell aging and enzyme activities was studied in rabbit, guinea-pig, hamster, rats (F344/N and SD), and mice (BALB/c and DBA/2). 2. The activities of six enzymes: glucose-6-phosphate dehydrogenase (G-6-PD), 6-phosphogluconate dehydrogenase (6-PGD), hexokinase (Hx), glutamate oxaloacetate transminase (GOT), lactate dehydrogenase (LDH) and acetylcholinesterase (AChE), were measured in the red cells of different ages which were obtained either by centrifugation or experimental anaemia. 3. Hx, AChE and GOT activities were much higher in younger red cells than in older cells, hence the activities of these enzymes may be used as an indicator of age of the cells.

摘要

研究了兔、豚鼠、仓鼠、大鼠（F344/N和SD）以及小鼠（BALB/c和DBA/2）红细胞衰老与酶活性之间的关系。2. 测定了六种酶的活性：葡萄糖-6-磷酸脱氢酶（G-6-PD）、6-磷酸葡萄糖酸脱氢酶（6-PGD）、己糖激酶（Hx）、谷草转氨酶（GOT）、乳酸脱氢酶（LDH）和乙酰胆碱酯酶（AChE），这些酶活性是在通过离心或实验性贫血获得的不同年龄红细胞中测定的。3. Hx、AChE和GOT活性在较年轻红细胞中比在较老红细胞中高得多，因此这些酶的活性可用作细胞年龄的指标。