Moos A B, Oughton J A, Kerkvliet N I
College of Veterinary Medicine, Oregon State University, Corvallis 97331-4801, USA.
Toxicol Lett. 1997 Feb 7;90(2-3):145-53. doi: 10.1016/s0378-4274(96)03838-6.
Recent studies in mice have demonstrated that TNF plays a critical role in mediating the TCDD-induced enhanced inflammatory response to intraperitoneal (i.p.) sheep red blood cells. The current studies were designed to evaluate the effects of TCDD on TNF production by ex-vivo peritoneal cells and a peritoneal macrophage cell line (IC-21) stimulated with LPS. In support of the hypothesis that TCDD can act directly on the peritoneal macrophage to increase TNF production, following pretreatment with TCDD, both ex-vivo peritoneal cells and IC-21 cells produced increased levels of bioactive TNF when stimulated with LPS. Flow cytometric analyses of IC-21 cells indicate that TCDD exposure increases intracellular production and secretion of TNF but does not alter levels of membrane associated TNF.
最近在小鼠身上进行的研究表明，肿瘤坏死因子（TNF）在介导2,3,7,8-四氯二苯并对二恶英（TCDD）诱导的对腹腔注射绵羊红细胞增强的炎症反应中起关键作用。当前的研究旨在评估TCDD对用脂多糖（LPS）刺激的离体腹膜细胞和腹膜巨噬细胞系（IC-21）产生TNF的影响。为支持TCDD可直接作用于腹膜巨噬细胞以增加TNF产生这一假说，在用TCDD预处理后，离体腹膜细胞和IC-21细胞在用LPS刺激时均产生了更高水平的生物活性TNF。对IC-21细胞的流式细胞术分析表明，暴露于TCDD会增加细胞内TNF的产生和分泌，但不会改变膜相关TNF的水平。
