Purkiss S F, Grahn M F, Williams N S
Academic Department of Surgery, St Bartholomew's and the Royal London School of Medicine and Dentistry, UK.
Surg Oncol. 1996 Aug;5(4):169-75. doi: 10.1016/s0960-7404(96)80040-5.
The p-glycoprotein export mechanism may have an effect on the cytotoxicity of chemotherapy or photodynamic therapy (PDT) by reducing cytotoxic drug or photosensitizer concentration within cells. In tissues over-expressing this protein, modulation with verapamil (an antagonist of p-glycoprotein) may be useful in reversing this form of treatment resistance. This study examined the bioactivity of the interaction of photodynamic therapy using Haematoporphyrin derivative (HpD), chemotherapy and the response modifier verapamil. Multicellular spheroids derived from the human colorectal cancer line HRT 18 were used in vitro and bioactivity assessed using growth retardation. Bioactivity was observed to be greatest when all three agents and light irradiation were combined. This application may be clinically useful in the treatment of colorectal carcinoma by improving the efficacy of PDT using HpD.
P-糖蛋白外排机制可能通过降低细胞内细胞毒性药物或光敏剂浓度，对化疗或光动力疗法（PDT）的细胞毒性产生影响。在过度表达该蛋白的组织中，用维拉帕米（一种P-糖蛋白拮抗剂）进行调节可能有助于逆转这种形式的治疗抗性。本研究检测了使用血卟啉衍生物（HpD）的光动力疗法、化疗与反应调节剂维拉帕米相互作用的生物活性。体外使用源自人结肠癌细胞系HRT 18的多细胞球体，并通过生长抑制评估生物活性。当三种药物与光照联合使用时，观察到生物活性最强。这种应用通过提高使用HpD的PDT疗效，可能在临床治疗结肠癌中具有实用价值。
