Ilagan A B, Forer A
Department of Biology, York University, Downsview, Ontario, Canada.
Cell Motil Cytoskeleton. 1997;36(3):266-75. doi: 10.1002/(SICI)1097-0169(1997)36:3<266::AID-CM7>3.0.CO;2-5.
Ultraviolet (UV)-microbeam irradiation of a single kinetochore during anaphase generally causes all 6 of the half-bivalents in the cell to stop poleward motion within 1 min after the irradiation. The half-bivalents regain movement after remaining stopped for an average of 8.7 min, through different pairs in the same cell can resume at different times. Once movement resumes they usually continue movement until they reach the poles. As controls, to see if the effect is due to alteration of the kinetochore, we irradiated spindle fibers and chromosome arms using the same doses and wavelengths as for kinetochore irradiation. After spindle fiber irradiation, only the half-bivalent associated with the irradiated spindle fiber and its partner stop moving poleward while the other half-bivalents in the same cell are not affected. After irradiation of a chromosome arm, the movement of the two partner half-bivalents associated with irradiated arm either slowed or moved with unchanged velocity; no other half-bivalents in the cell were affected. Therefore, only irradiation of a kinetochore stops the movement of all the half-bivalents in the same cell. We suggest that the irradiated kinetochore sends a "stop" signal to the other kinetochores in the same cell.
在后期对单个动粒进行紫外线（UV）微束照射，通常会使细胞中所有6条半二价体在照射后1分钟内停止向极运动。半二价体在停止平均8.7分钟后恢复运动，同一细胞中的不同对半二价体恢复运动的时间可能不同。一旦恢复运动，它们通常会继续移动直到到达两极。作为对照，为了确定这种效应是否是由于动粒的改变引起的，我们使用与照射动粒相同的剂量和波长照射纺锤体纤维和染色体臂。照射纺锤体纤维后，只有与被照射纺锤体纤维及其配对物相关的半二价体停止向极运动，而同一细胞中的其他半二价体不受影响。照射染色体臂后，与被照射臂相关的两个配对半二价体的运动要么减慢，要么速度不变；细胞中的其他半二价体均未受到影响。因此，只有照射动粒才会使同一细胞中的所有半二价体停止运动。我们认为，被照射的动粒会向同一细胞中的其他动粒发送“停止”信号。
