Jensen R E, Kinnally K W
Department of Cell Biology and Anatomy, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA.
J Bioenerg Biomembr. 1997 Feb;29(1):3-10. doi: 10.1023/a:1022470303365.
Mitochondrial biogenesis requires the import of hundreds of different proteins from the cytosol. Protein import into mitochondria is a multistep pathway that includes recognition of precursor proteins by machinery both in the cytoplasm and on the mitochondrial surface, translocation of the precursor across one or both mitochondrial membranes, and folding of the protein after its import into the organelle. Over the past several years, many components of the import machinery have been identified using both biochemical and genetic methods. Recently, significant progress has been made determining the function of some of these import proteins. One purpose of this minireview is to summarize our current understanding of the import pathway, and to introduce the topics of the minireviews that will follow. The other goal of this minireview is to discuss recent findings suggesting that proteins are translocated across both the mitochondrial inner and outer membranes through aqueous channels.
线粒体生物合成需要从细胞质中导入数百种不同的蛋白质。蛋白质导入线粒体是一个多步骤过程，包括细胞质和线粒体外膜上的机制对前体蛋白的识别、前体蛋白穿过一层或两层线粒体膜的转运，以及蛋白导入细胞器后的折叠。在过去几年中，通过生化和遗传学方法已鉴定出导入机制的许多组成部分。最近，在确定其中一些导入蛋白的功能方面取得了重大进展。本微型综述的一个目的是总结我们目前对导入途径的理解，并介绍后续微型综述的主题。本微型综述的另一个目标是讨论最近的研究结果，这些结果表明蛋白质通过水通道穿过线粒体内外膜。
