Groody E P
Gene-Trak, Hopkinton, MA 01747, USA.
Mol Biotechnol. 1996 Dec;6(3):323-7. doi: 10.1007/BF02761710.
The detection of foodborne microorganisms has traditionally been done using microbiologically based methods. Such "gold standard" methods are generally reliable but have the disadvantages of being labor intensive, subjective, and time consuming. Over the last several years, the development of DNA probe-based methods has simplified the methods used to detect organisms such as Salmonella, Listeria, and E. coli by targeting the unique DNA or RNA sequences of these organisms using DNA probes and nonradioactive detection.
传统上，食源微生物的检测是使用基于微生物学的方法来进行的。这类“金标准”方法通常是可靠的，但具有劳动强度大、主观性强和耗时的缺点。在过去几年中，基于DNA探针的方法的发展，通过使用DNA探针和非放射性检测来靶向这些生物体独特的DNA或RNA序列，简化了用于检测沙门氏菌、李斯特菌和大肠杆菌等生物体的方法。
