Fitts R, Diamond M, Hamilton C, Neri M
Appl Environ Microbiol. 1983 Nov;46(5):1146-51. doi: 10.1128/aem.46.5.1146-1151.1983.
We have developed a DNA-DNA hybridization test for the presence of Salmonella spp. in foods. This test requires an initial pre-enrichment of food samples in nutrient broth but does not require selective enrichment. Samples of food cultures are collected on membrane filters and assayed by molecular hybridization to labeled probes. The probes consist of DNA sequences which are unique to the genus Salmonella and are widely distributed in the genus. A diverse panel of foods was assayed successfully by this methodology.
我们开发了一种用于检测食品中沙门氏菌属存在情况的DNA-DNA杂交试验。该试验需要先将食品样本在营养肉汤中进行预富集，但不需要选择性富集。将食品培养物样本收集在膜滤器上，并用标记探针进行分子杂交分析。这些探针由沙门氏菌属特有的DNA序列组成，且在该属中广泛分布。通过这种方法成功检测了多种不同的食品。
