Knight J S, Madueño F, Barnes S A, Gray J C
School of Biological Sciences, University of Manchester, UK.
Mol Biotechnol. 1996 Dec;6(3):335-45. doi: 10.1007/BF02761712.
The levels of individual photosynthetic proteins can be independently decreased by the Agrobacterium-mediated transformation of plants with antisense RNA constructs. Protocols for the introduction of such constructs into Agrobacterium, the Agrobacterium-mediated transformation of tobacco leaf disks, and the screening and analysis of the transgenic plants produced are described.
通过用反义RNA构建体对植物进行农杆菌介导的转化，可以独立降低单个光合蛋白的水平。本文描述了将此类构建体导入农杆菌、农杆菌介导的烟草叶盘转化以及对所产生的转基因植物进行筛选和分析的方法。
