Bevan M
Nucleic Acids Res. 1984 Nov 26;12(22):8711-21. doi: 10.1093/nar/12.22.8711.
A vector molecule for the efficient transformation of higher plants has been constructed with several features that make it efficient to use. It utilizes the trans acting functions of the vir region of a co-resident Ti plasmid in Agrobacterium tumefaciens to transfer sequences bordered by left and right T-DNA border sequences into the nuclear genome of plants. The T-region contains a dominant selectable marker gene that confers high levels of resistance to kanamycin, and a lac alpha-complementing region from M13mp19 that contains several unique restriction sites for the positive selection of inserted DNA.
一种用于高效转化高等植物的载体分子已被构建出来，它具有几个使其易于使用的特性。它利用根癌土壤杆菌中共存的Ti质粒的vir区的反式作用功能，将由左右T-DNA边界序列界定的序列转移到植物的核基因组中。T区包含一个赋予对卡那霉素高水平抗性的显性选择标记基因，以及来自M13mp19的一个乳糖α-互补区域，该区域包含几个用于阳性选择插入DNA的独特限制性酶切位点。
