Musette P, Benveniste O, Lim A, Bequet D, Kourilsky P, Dormont D, Gachelin G
Unité de Biologie moléculaire du Gène, INSERM U277, Institut Pasteur, Paris, France.
Res Immunol. 1996 Sep;147(7):435-41. doi: 10.1016/s0923-2494(97)84408-2.
Using two independent PCR-based quantification techniques, we have determined the levels of IL1 beta, IL2, IL4, IL6, IL10, IFN gamma and TNF alpha mRNA in multiple sclerosis patients at the moment of diagnosis of the disease and prior to any immunosuppressive treatment. These patients exhibit markedly reduced IL2 and IL10 mRNA expression accompanied by decreased levels of TNF alpha mRNA. Our results add to the evidence that IL10 plays a role in multiple sclerosis and suggest that decreased production of this interleukin allows the proliferation of autoreactive T cells at the onset of the disease.
我们运用两种基于聚合酶链反应（PCR）的独立定量技术，测定了多发性硬化症患者在疾病诊断时及任何免疫抑制治疗之前，白细胞介素1β（IL1β）、白细胞介素2（IL2）、白细胞介素4（IL4）、白细胞介素6（IL6）、白细胞介素10（IL10）、干扰素γ（IFNγ）和肿瘤坏死因子α（TNFα）的信使核糖核酸（mRNA）水平。这些患者表现出白细胞介素2和白细胞介素10的信使核糖核酸表达显著降低，同时肿瘤坏死因子α的信使核糖核酸水平也降低。我们的结果进一步证明白细胞介素10在多发性硬化症中发挥作用，并表明这种白细胞介素产生的减少使得自身反应性T细胞在疾病发作时得以增殖。
