Ghamsari S M, Taguchi K, Abe N, Acorda J A, Sato M, Yamada H
Department of Veterinary Surgery, School of Veterinary Medicine, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, Japan.
Vet Surg. 1997 Mar-Apr;26(2):114-20. doi: 10.1111/j.1532-950x.1997.tb01473.x.
The purpose of this study was to evaluate the effect of low-level laser therapy (LLLT) on sutured wounds of the teat in dairy cattle.
By using the Latin square design, the effect of LLLT was evaluated by radiography, measurement of microcirculation flow, histopathology, tensiometry, and hydroxyproline analysis.
Sixteen teats of four dairy cattle.
Full thickness wounds were made on the cranial surface of the teats. Teats were distributed into four groups; group A and B wounds were closed with a Gambee pattern, group C and D wounds were closed with three-layers of continuous suture pattern. Group B and D wounds were treated with 3.64 J/cm2 of LLLT using a helium-neon system continuous wave (632.8 nm) output of 8.5 nW.
The teat wall in non-LLLT groups was significantly thicker than in LLLT groups on day 7, 14 and 21. The mean blood flow differences between control and sutured sites in LLLT groups were significantly lower than those in non-LLLT groups. The morphology of the epidermis in LLLT groups more closely resembled the normal epidermis than that of non-LLLT groups. Collagen fibers in LLLT groups were denser, thicker, better arranged and more continuous with existing collagen fibers than those in non-LLLT groups. The mean tensile strength was significantly greater in LLLT groups than in non-LLLT groups.
The LLLT affects various aspects of the healing process, including minimizing inflammation, formation of edema, improvement of skin regeneration and enhancement of collagen synthesis.
The LLLT could accelerate healing of sutured wounds of the teat in dairy cattle.
本研究旨在评估低强度激光疗法（LLLT）对奶牛乳头缝合伤口的影响。
采用拉丁方设计，通过放射摄影、微循环血流测量、组织病理学、张力测定和羟脯氨酸分析来评估LLLT的效果。
四头奶牛的16个乳头。
在乳头的颅面制作全层伤口。乳头被分为四组；A组和B组伤口采用Gambee缝合法缝合，C组和D组伤口采用三层连续缝合法缝合。B组和D组伤口使用氦氖系统连续波（632.8纳米）、输出功率8.5纳瓦、剂量为3.64焦/平方厘米的LLLT进行治疗。
在第7天、14天和21天，非LLLT组的乳头壁明显比LLLT组厚。LLLT组对照部位与缝合部位之间的平均血流差异明显低于非LLLT组。LLLT组表皮的形态比非LLLT组更接近正常表皮。与非LLLT组相比，LLLT组的胶原纤维更致密、更厚、排列更好且与现有胶原纤维更连续。LLLT组的平均抗张强度明显高于非LLLT组。
LLLT影响愈合过程的各个方面，包括将炎症降至最低、水肿形成、皮肤再生改善和胶原合成增强。
LLLT可加速奶牛乳头缝合伤口的愈合。
