Way A L, Schuler A M, Killian G J
Dairy Breeding Research Center, Pennsylvania State University, University Park 16802, USA.
J Reprod Fertil. 1997 Jan;109(1):95-101. doi: 10.1530/jrf.0.1090095.
The objective of this study was to determine the effects of oviductal fluid on sperm binding and fertilization in vitro when either bovine oocytes or both spermatozoa and oocytes are incubated in fluid from different regions of the oviduct and at different stages of the oestrous cycle. Oocytes, or oocytes and spermatozoa, were incubated in nonluteal and luteal oviductal fluid obtained from the whole, isthmic or ampullary oviduct of dairy cows. Gametes were washed and coincubated in fertilization medium for 18 h, and then observed for sperm binding and penetration. More spermatozoa bound to oocytes that were preincubated in nonluteal oviductal fluid than to those that were incubated in luteal oviductal fluid; however, there were no differences in fertilization rates between the two treatments. More spermatozoa bound to oocytes incubated in isthmic than in ampullary oviductal fluid. However, if both gametes were incubated in oviductal fluid before their coincubation, binding was higher when both gametes were incubated in ampullary oviductal fluid. By reducing the time of coincubation of spermatozoa with the oocytes, differences in fertilization rates were seen among the various oviductal fluid treatments. At 14 and 16 h of coincubation, more oocytes were fertilized if spermatozoa were preincubated in isthmic oviductal fluid and oocytes were preincubated in ampullary oviductal fluid than if both gametes were incubated in whole or ampullary oviductal fluid. Because both sperm binding to the zona pellucida and fertilization rates were greater in treatments in which gametes were exposed to regional oviductal fluid than when they were incubated in whole oviductal fluid, it is concluded that oviductal fluid from different regions of the bovine oviduct may play different roles in facilitating fertilization.
本研究的目的是，当牛卵母细胞或精子与卵母细胞二者均在来自输卵管不同区域且处于发情周期不同阶段的液体中孵育时，确定输卵管液对体外精子结合及受精的影响。将卵母细胞或卵母细胞与精子在从奶牛整个输卵管、峡部或壶腹部获取的非黄体期和黄体期输卵管液中孵育。配子经洗涤后在受精培养基中共孵育18小时，然后观察精子的结合和穿透情况。与在黄体期输卵管液中孵育的卵母细胞相比，更多精子与在非黄体期输卵管液中预孵育的卵母细胞结合；然而，两种处理的受精率并无差异。与在壶腹部输卵管液中孵育的卵母细胞相比，更多精子与在峡部输卵管液中孵育的卵母细胞结合。然而，如果在共孵育前将两种配子均在输卵管液中孵育，当两种配子均在壶腹部输卵管液中孵育时，结合率更高。通过缩短精子与卵母细胞的共孵育时间，在不同输卵管液处理之间观察到了受精率的差异。在共孵育14和16小时时，与两种配子均在整个或壶腹部输卵管液中孵育相比，如果精子在峡部输卵管液中预孵育且卵母细胞在壶腹部输卵管液中预孵育，则更多卵母细胞受精。由于与配子在整个输卵管液中孵育相比，当配子暴露于局部输卵管液时，精子与透明带的结合及受精率均更高，因此得出结论，牛输卵管不同区域的输卵管液在促进受精方面可能发挥不同作用。
