Anderson S H, Killian G J
Department of Dairy and Animal Science, Pennsylvania State University, University Park 16802.
Biol Reprod. 1994 Oct;51(4):795-9. doi: 10.1095/biolreprod51.4.795.
The effect of macromolecules from oviductal conditioned medium (CM) on sperm motility and capacitation was studied. Sperm pooled from three bulls was incubated in either luteal isthmic CM, luteal ampullary CM, estrual isthmic CM, estrual ampullary CM, or control medium (no CM) for 4 h. Sperm capacitation and motility were assessed at 10 min and 4 h. Estrual isthmic CM capacitated significantly more spermatozoa at 4 h than estrual ampullary CM or control medium. CM also affected lateral head movement (ALH) and beat cross-frequency (BCF) of sperm. In a second experiment, the glycosaminoglycan (GAG) content of the different types of oviductal CM was quantified. Estrual isthmic CM contained more GAG than estrual ampullary CM. Among luteal samples, no difference in GAG concentration between the isthmic and ampullary CM was found. Heat treatment (100 degrees C) of oviductal CM before coincubation with sperm significantly reduced, but did not eliminate, the capacitating ability. Because heat treatment denatures proteins and decreases the capacitating ability of certain GAG, we concluded that the capacitating effect of estrual isthmic CM may be associated with proteins, GAG, and proteoglycans in the CM. Isthmic secretions may play a major role during in vivo sperm capacitation, given that bovine spermatozoa may reside in the oviduct isthmus for up to 18 h before fertilization.
研究了输卵管条件培养基（CM）中的大分子对精子活力和获能的影响。将来自三头公牛的精子分别在黄体期峡部CM、黄体期壶腹部CM、发情期峡部CM、发情期壶腹部CM或对照培养基（无CM）中孵育4小时。在10分钟和4小时时评估精子获能和活力。发情期峡部CM在4小时时使精子获能的数量显著多于发情期壶腹部CM或对照培养基。CM还影响精子的侧向头部运动（ALH）和摆动交叉频率（BCF）。在第二个实验中，对不同类型的输卵管CM中的糖胺聚糖（GAG）含量进行了定量。发情期峡部CM中的GAG含量高于发情期壶腹部CM。在黄体期样本中，峡部CM和壶腹部CM之间的GAG浓度没有差异。在与精子共同孵育之前对输卵管CM进行热处理（100℃）显著降低但并未消除其获能能力。由于热处理会使蛋白质变性并降低某些GAG的获能能力，我们得出结论，发情期峡部CM的获能作用可能与CM中的蛋白质、GAG和蛋白聚糖有关。鉴于牛精子在受精前可能在输卵管峡部停留长达18小时，峡部分泌物可能在体内精子获能过程中起主要作用。
