在天冬酰胺降解方面发生改变的菜豆根瘤菌突变体的分离与鉴定
Isolation and characterization of Rhizobium etli mutants altered in degradation of asparagine.
作者信息
Huerta-Zepeda A, Ortuño L, Du Pont G, Durán S, Lloret A, Merchant-Larios H, Calderón J
机构信息
Departamento de Biotecnología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, México D.F.
出版信息
J Bacteriol. 1997 Mar;179(6):2068-72. doi: 10.1128/jb.179.6.2068-2072.1997.
Abstract
Rhizobium etli mutants unable to grow on asparagine as the nitrogen and carbon source were isolated. Two kinds of mutants were obtained: AHZ1, with very low levels of aspartase activity, and AHZ7, with low levels of asparaginase and very low levels of aspartase compared to the wild-type strain. R. etli had two asparaginases differentiated by their thermostabilities, electrophoretic mobilities, and modes of regulation. The AHZ mutants nodulated as did the wild-type strain and had nitrogenase levels similar to that of the wild-type strain.
摘要
分离出了不能以天冬酰胺作为氮源和碳源生长的费氏中华根瘤菌突变体。获得了两种突变体：AHZ1，天冬氨酸酶活性极低；AHZ7，与野生型菌株相比，天冬酰胺酶水平低且天冬氨酸酶水平极低。费氏中华根瘤菌有两种天冬酰胺酶，可通过它们的热稳定性、电泳迁移率和调节方式来区分。AHZ突变体与野生型菌株一样能够结瘤，并且固氮酶水平与野生型菌株相似。
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