Muller M M, Webster R E
Department of Biochemistry, Duke University Medical Center, Durham, North Carolina 27710, USA.
J Bacteriol. 1997 Mar;179(6):2077-80. doi: 10.1128/jb.179.6.2077-2080.1997.
Sequence analysis showed that the cyd operon is immediately upstream of the tol-pal region. Northern (RNA) blot analysis demonstrated that the transcript for the cyd operon terminates just before the promoter for transcription of the tol genes. The cyd transcript contains cydA cydB followed by two open reading frames: orfC, encoding a 37-residue peptide, and orfD, encoding a 97-residue peptide. Both OrfC and OrfD are synthesized in minicells.
序列分析表明,cyd操纵子紧邻tol-pal区域的上游。Northern(RNA)印迹分析表明,cyd操纵子的转录本在tol基因转录启动子之前终止。cyd转录本包含cydA、cydB,随后是两个开放阅读框:orfC,编码一个37个氨基酸残基的肽,以及orfD,编码一个97个氨基酸残基的肽。OrfC和OrfD均在小细胞中合成。