Chen C, Hughes J D, Mattar S G, Ku D N, Lumsden A B
Department of Surgery, Veterans Affairs Medical Center, Decatur, Georgia 30033, USA.
J Surg Res. 1997 Jan;67(1):106-12. doi: 10.1006/jsre.1996.4922.
In an effort to characterize intimal hyperplastic lesions, we have undertaken a time-course study in a canine endarterectomy model of intimal hyperplasia. Twenty dogs underwent surgical endarterectomies of the carotid arteries. A total of 23 of 27 (85%) injured arteries were patent, which consisted of 6, 8, 5, and 4 arteries found to be patent at 1, 2, 5, and 11 weeks, respectively. Measurable intimal thickening developed at 1 week (0.08 +/- 0.01 mm) and at 2 weeks (0.13 +/- 0.02 mm), maximized at 5 weeks (0.29 +/- 0.03 mm), and subsided at 11 weeks (0.21 +/- 0.01 mm) after injury. Endothelial cells covering intimal hyperplastic tissues were seen only at 11 weeks. The intimal cell proliferation rate reached a maximum of 24% at 1 week, decreased dramatically at 2 weeks, and remained at low levels but higher than baseline levels at 5 and 11 weeks. Extracellular matrix (ECM) content accounted for 29% of total intimal volume at 1 week after endarterectomy and increased to 37, 40, and 47% at 2, 5, and 11 weeks, respectively. These data demonstrate that maximum intimal cell proliferation occurs at 1 week and maximum intimal hyperplasia at 5 weeks after arterial injury. Intimal ECM content increased with time after injury throughout the duration of this study. The uniform and consistent intimal lesion that was established in this large animal model is clinically relevant and can be used to study cellular and molecular mechanisms of restenosis and to evaluate therapeutic interventions.
为了描述内膜增生性病变的特征，我们在犬内膜增生的动脉内膜切除术模型中进行了一项时间进程研究。20只犬接受了颈动脉的手术内膜切除术。27条受损动脉中有23条（85%）保持通畅，其中分别有6条、8条、5条和4条动脉在术后1周、2周、5周和11周时被发现通畅。可测量的内膜增厚在损伤后1周（0.08±0.01毫米）和2周（0.13±0.02毫米）时出现，在5周时达到最大值（0.29±0.03毫米），并在11周时消退（0.21±0.01毫米）。覆盖内膜增生组织的内皮细胞仅在11周时可见。内膜细胞增殖率在1周时最高达到24%，在2周时急剧下降，并在5周和11周时保持在低水平但高于基线水平。动脉内膜切除术后1周时，细胞外基质（ECM）含量占内膜总体积的29%，在2周、5周和11周时分别增加到37%、40%和47%。这些数据表明，动脉损伤后1周时内膜细胞增殖达到最大值，5周时内膜增生达到最大值。在本研究的整个过程中，内膜ECM含量随损伤后的时间增加而增加。在这个大型动物模型中建立的均匀一致的内膜病变具有临床相关性，可用于研究再狭窄的细胞和分子机制以及评估治疗干预措施。
