Chen C, Coyle K A, Hughes J D, Lumsden A B, Ku D N
Department of Surgery, Emory University School of Medicine, Atlanta, Georgia, USA.
Cardiovasc Surg. 1997 Apr;5(2):161-8. doi: 10.1016/s0967-2109(96)00086-5.
The purpose of this study was to evaluate a technique that accelerates intimal hyperplasia by reduction of blood flow. Bilateral endarterectomies were performed in both femoral and carotid arteries in six dogs. One week later, all animals underwent banding of an artery distal to the injured region to reduce the blood flow by 50%. The contralateral injured arteries served as controls. At 11 weeks, the specimens were harvested and analyzed. Five of 12 (42%) of the flow-restricted arteries and nine of 12 (75%) of the non-flow-restricted arteries were patent at 11 weeks (P<0.05). Marked stenotic intimal hyperplastic lesions developed in the flow-restricted arteries (69% stenosis) as compared with the non-flow-restricted arteries (37% stenosis). Mean(s.d.) intimal thickness, intimal areas, and intimal/medial area ratio were 0.52(0.19) mm, 3.17(1.11) mm2, and 1.12(0.33)%, respectively, in the flow-restricted arteries. Their counterparts in the non-flow-restricted arteries were 0.21(0.09) mm, 1.70(1.09) mm2, and 0.58(0.14)%, respectively (P<0.05). Extracellular matrix comprised 48% of total intimal volumes in the flow-restricted arteries. Cell proliferation and occluded arteries were also characterized. These data demonstrate that reduction of blood flow significantly accelerated intimal hyperplasia and occlusion rates in endarterectomized arteries. Advanced intimal hyperplastic lesions (>50% stenosis) possess a high extracellular matrix content. This new animal model is a reliable generator of advanced stenotic lesions in a relatively short time period and can be used to study biologic mechanisms of stenosis and evaluate therapeutic interventions.
本研究的目的是评估一种通过减少血流来加速内膜增生的技术。对6只狗的股动脉和颈动脉进行双侧内膜切除术。一周后，所有动物均对损伤区域远端的动脉进行结扎，以减少50%的血流。对侧受伤动脉作为对照。在11周时，采集标本并进行分析。在11周时，12条血流受限动脉中有5条（42%）保持通畅，12条非血流受限动脉中有9条（75%）保持通畅（P<0.05）。与非血流受限动脉（狭窄37%）相比，血流受限动脉出现了明显的狭窄性内膜增生病变（狭窄69%）。血流受限动脉的平均（标准差）内膜厚度、内膜面积和内膜/中膜面积比分别为0.52（0.19）mm、3.17（1.11）mm²和1.12（0.33）%。非血流受限动脉的相应数据分别为0.21（0.09）mm、1.70（1.09）mm²和0.58（0.14）%（P<0.05）。细胞外基质占血流受限动脉内膜总体积的48%。还对细胞增殖和闭塞动脉进行了表征。这些数据表明，减少血流可显著加速内膜切除术后动脉的内膜增生和闭塞率。晚期内膜增生病变（狭窄>50%）具有较高的细胞外基质含量。这种新的动物模型能够在相对较短的时间内可靠地产生晚期狭窄病变，可用于研究狭窄的生物学机制并评估治疗干预措施。
