Yamada H, Hirai K, Miyamasu M, Iikura M, Misaki Y, Shoji S, Takaishi T, Kasahara T, Morita Y, Ito K
Department of Medicine and Physical Therapy, University of Tokyo School of Medicine, Japan.
Biochem Biophys Res Commun. 1997 Feb 13;231(2):365-8. doi: 10.1006/bbrc.1997.6100.
We studied the effect of eotaxin, a novel eosinophil-active CC chemokine with high target cell specificity, on human basophils. Eotaxin induced higher levels of chemotactic response with a lower ED50 compared with RANTES in basophils; half-maximal migration occurred at a concentration of approximately 3 nM. On the other hand, it exerted only a marginal effect on either histamine release or leukotriene C4 generation. In addition, nested PCR amplification experiments revealed the expression of CC CKR3, a putative receptor for eotaxin, on basophils. Since accumulation of both basophils and eosinophils is an important aspect of allergic inflammation, eotaxin potentially plays a pathogenic role in allergic disorders by inducing migration of both of these cell types.
我们研究了嗜酸性粒细胞趋化因子（一种具有高靶细胞特异性的新型嗜酸性粒细胞活性CC趋化因子）对人嗜碱性粒细胞的作用。与RANTES相比，嗜酸性粒细胞趋化因子在嗜碱性粒细胞中诱导出更高水平的趋化反应，且ED50更低；在浓度约为3 nM时出现半数最大迁移。另一方面，它对组胺释放或白三烯C4生成仅产生轻微影响。此外，巢式PCR扩增实验显示嗜碱性粒细胞上存在CC CKR3（一种推测的嗜酸性粒细胞趋化因子受体）的表达。由于嗜碱性粒细胞和嗜酸性粒细胞的聚集是过敏性炎症的一个重要方面，嗜酸性粒细胞趋化因子可能通过诱导这两种细胞类型的迁移在过敏性疾病中发挥致病作用。
