Warren K S, Pelley R P, Mahmoud A A
Am J Trop Med Hyg. 1977 Sep;26(5 Pt 1):957-62. doi: 10.4269/ajtmh.1977.26.957.
The effect of curative treatment of murine schistosomiasis mansoni on resistance to reinfection and on granulomatous hypersensitivity following reinfection was studied. The sera of mice with light chronic Schistosoma mansoni infection of 32 weeks duration were tested by immunodiffusion analysis with soluble egg antigens for a precipitin band containing an antigen specific for eggs in order to identify bisexual infections. A group of the 32 week bisexually-infected mice were treated with niridazole, as was a similar group of uninfected mice. Six weeks later (at 38 weeks) the previously infected mice were injected subcutaneously with 30 cercariae and the uninfected mice with 20 cercariae. On perfusion 12 weeks later the secondarily infected mice had a percentage worm yield following infection of 16 while that in the primarily infected mice was 60. Twelve weeks after reinfection (at 50 weeks), the previously infected mice had moderate hepatosplenic disease similar to the mice which had remained constantly infected for 50 weeks, and formed relatively small granulomas around eggs injected into their pulmonary microvasculature. In spite of much lower liver egg counts, particularly severe hepatosplenic disease was seen in the previously uninfected mice. When eggs were injected into their lungs, however, very large granulomas were observed. These experiments suggest that both immunity and modulation of immunopathology are residual after curative treatment.
研究了小鼠曼氏血吸虫病的根治性治疗对再感染抵抗力以及再感染后肉芽肿超敏反应的影响。用可溶性虫卵抗原通过免疫扩散分析检测持续32周的轻度慢性曼氏血吸虫感染小鼠的血清，以寻找含有虫卵特异性抗原的沉淀带，从而确定两性感染情况。一组感染32周的两性感染小鼠和一组类似的未感染小鼠用硝唑咪进行治疗。六周后（38周时），给先前感染的小鼠皮下注射30条尾蚴，给未感染的小鼠注射20条尾蚴。12周后灌注时，再次感染的小鼠感染后的虫体产出率为16%，而初次感染小鼠的虫体产出率为60%。再感染12周后（50周时），先前感染的小鼠出现了与持续感染50周的小鼠相似的中度肝脾疾病，并在注入其肺微血管的虫卵周围形成了相对较小的肉芽肿。尽管肝内虫卵计数低得多，但先前未感染的小鼠出现了特别严重的肝脾疾病。然而，当向它们的肺部注入虫卵时，观察到了非常大的肉芽肿。这些实验表明，根治性治疗后免疫和免疫病理学调节均有残留。
