Antinori S, Versaci C, Dani G, Antinori M, Pozza D, Selman H A
R.A.P.R.U.I Day Hospital, Rome, Italy.
Hum Reprod. 1997 Feb;12(2):286-91. doi: 10.1093/humrep/12.2.286.
Between July 1995 and May 1996, 36 patients with non-obstructive azoospermia of secretory origin underwent intracytoplasmic injection of spermatids. A previous histological biopsy was performed on all patients: 15 had spermatogenic arrest, a further 13 had Sertoli cell-only syndrome, and the remaining eight had post-cryptorchidism tubal atrophy. The ejaculate was duly examined and a complete absence of spermatozoa and spermatids was confirmed, with only bacteria and debris being found. Testicular sperm extraction (TESE) was then performed. In 19 out of 36 cases round spermatids only were found, while elongated spermatids were found in the remaining 17. Both round and elongated spermatids were isolated and used for injection. A total of 135 oocytes at metaphase II were recovered from 19 partners and injected with round spermatids, while 123 mature oocytes from 17 partners were injected with elongated spermatids. The number of oocytes fertilized, as judged by the presence of two pronuclei, was 75 (55.5%) and 71 (57.7%) respectively. By 34 h after injection, the number of embryos which had cleaved to the 2-cell stage was 56 (74.6%) with round spermatids and 55 (77.4%) with elongated spermatids. All cleaved embryos were transferred into the uterus of the partners. Clinical pregnancies were established in two cases of round spermatid cycles (10.5%) (both are still ongoing), and three cases of elongated spermatid cycles (17.6%) (two are still ongoing; one was lost after 8 weeks of gestation). Chromosomal analysis showed that all fetuses had a normal karyotype (three male and one female) with no chromosomal abnormalities.
1995年7月至1996年5月期间，36例分泌性来源的非梗阻性无精子症患者接受了精子胞浆内注射。所有患者均预先进行了组织学活检：15例为生精停滞，另外13例为唯支持细胞综合征，其余8例为隐睾后输卵管萎缩。对精液进行了适当检查，确认完全没有精子和精子细胞，仅发现细菌和碎片。然后进行了睾丸精子提取（TESE）。36例中有19例仅发现圆形精子细胞，其余17例发现了长形精子细胞。圆形和长形精子细胞均被分离出来用于注射。从19名配偶中回收了总共135个处于减数分裂中期II的卵母细胞，并注射了圆形精子细胞，同时从17名配偶中回收了123个成熟卵母细胞，并注射了长形精子细胞。根据双原核的存在判断，受精的卵母细胞数量分别为75个（55.5%）和71个（57.7%）。注射后34小时，分裂至2细胞期的胚胎数量，注射圆形精子细胞的为56个（74.6%），注射长形精子细胞的为55个（77.4%）。所有分裂的胚胎均被转移至配偶的子宫内。圆形精子细胞周期中有2例（10.5%）成功建立临床妊娠（均仍在继续），长形精子细胞周期中有3例（17.6%）成功建立临床妊娠（2例仍在继续；1例在妊娠8周后流产）。染色体分析显示，所有胎儿的核型均正常（3男1女），无染色体异常。
