Ding M, Yuwen H, Mitchell F, Biswas R, Ndimbie O K, Farshid M
Laboratory of Hepatitis, Food and Drug Administration, Bethesda, Maryland 20892, USA.
Biochem Biophys Res Commun. 1997 Feb 24;231(3):606-9. doi: 10.1006/bbrc.1997.6159.
The nucleotide sequences of the 5' noncoding region of the GB virus C/hepatitis G virus (GBV-C/HGV) were determined in 18 isolates from the United States. Two genotypes have been classified based on the sequence heterogeneity within the 5' noncoding region of GBV-C/HGV. The most distantly related isolates between the two genotypes were 84.6% identical. Sequence identity of the isolates within a genotype was 95-99%. The 5' noncoding region of this virus contains four highly conserved domains. These conserved elements would facilitate the selection of optimal primers for the sensitive detection of GBV-C/HGV RNA by PCR. In addition, they suggest a crucial role for this region in viral replication and/or gene expression. Detection of genotypic variation among GBV-C/HGV infected individuals may provide further insight into the possible pathogenicity and into the transmission of the virus.
测定了来自美国的18株GB病毒C型/庚型肝炎病毒（GBV-C/HGV）5'非编码区的核苷酸序列。根据GBV-C/HGV 5'非编码区内的序列异质性，已将其分为两种基因型。两种基因型中亲缘关系最远的分离株的序列一致性为84.6%。同一基因型内分离株的序列一致性为95%-99%。该病毒的5'非编码区包含四个高度保守的结构域。这些保守元件将有助于选择最佳引物，通过聚合酶链反应（PCR）灵敏检测GBV-C/HGV RNA。此外，它们表明该区域在病毒复制和/或基因表达中起关键作用。检测GBV-C/HGV感染个体之间的基因型变异可能会进一步深入了解该病毒可能的致病性和传播情况。
