人类Edg2蛋白的分子克隆及其作为溶血磷脂酸功能性细胞受体的鉴定。
Molecular cloning of the human Edg2 protein and its identification as a functional cellular receptor for lysophosphatidic acid.
作者信息
An S, Dickens M A, Bleu T, Hallmark O G, Goetzl E J
机构信息
Department of Medicine, University of California Medical Center, San Francisco 94143-0711, USA.
出版信息
Biochem Biophys Res Commun. 1997 Feb 24;231(3):619-22. doi: 10.1006/bbrc.1997.6150.
A cDNA homologous to that encoding sheep Edg2 protein was cloned from a human lung cDNA library. The full-length sequence encodes a 364-amino acid protein which belongs to the superfamily of guanine nucleotide-binding (G) protein-coupled receptors. Human Edg2 mRNA is widely distributed in human tissues with the highest abundance in brain. HEK293 cells expressing the human Edg2 protein showed an elevated response to lysophosphatidic acid (LPA) in a serum response element reporter gene assay, which was LPA concentration-dependent and specific to LPA compared to other lysophospholipids. Over-expression of human Edg2 in CHO cells correlated with increases in specific binding of [3H]-LPA. Recently, the mouse counterpart of Edg2 protein also was identified as a receptor for LPA (Hecht et al., 1996, J. Cell Biol. 135, 1071). Therefore, it is concluded that the human Edg2 protein functions as a cellular receptor for LPA.
从人肺cDNA文库中克隆出与编码绵羊Edg2蛋白的cDNA同源的序列。其全长序列编码一个364个氨基酸的蛋白质，该蛋白质属于鸟嘌呤核苷酸结合（G）蛋白偶联受体超家族。人Edg2 mRNA广泛分布于人体组织中，在脑中含量最高。在血清反应元件报告基因分析中，表达人Edg2蛋白的HEK293细胞对溶血磷脂酸（LPA）的反应增强，该反应呈LPA浓度依赖性，且与其他溶血磷脂相比对LPA具有特异性。在CHO细胞中过表达人Edg2与[3H]-LPA特异性结合增加相关。最近，Edg2蛋白的小鼠对应物也被鉴定为LPA的受体（Hecht等人，1996年，《细胞生物学杂志》135卷，1071页）。因此，可以得出结论，人Edg2蛋白作为LPA的细胞受体发挥作用。
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