STRL22基因的克隆，该基因是一种新的人类基因，编码一种与趋化因子受体相关的G蛋白偶联受体，定位于6号染色体q27区。

Cloning of STRL22, a new human gene encoding a G-protein-coupled receptor related to chemokine receptors and located on chromosome 6q27.

作者信息

Liao F, Lee H H, Farber J M

机构信息

Laboratory of Clinical Investigation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA.

出版信息

Genomics. 1997 Feb 15;40(1):175-80. doi: 10.1006/geno.1996.4544.

DOI:10.1006/geno.1996.4544

PMID:9070937

Abstract

Using PCR with pools of primers based on conserved sequences in chemokine receptors, we have cloned a human member of the G-protein-coupled receptor gene family. The new gene, which we have named STRL22, is predicted to encode a receptor related to chemokine receptors, including IL8RA (CXCR1), IL8RB (CXCR2), and CXCR4/fusin, and to the orphan receptors EBI1 and BLR1. Consistent with a role in leukocyte biology, STRL22 is expressed in lymphocytes and in lymphoid tissue. We have mapped STRL22 to chromosome 6q27. STRL22 cDNAs reflect alternative (or incomplete) mRNA processing in the 5'-nontranslated region, a phenomenon found in analysis of other chemoattractant receptor genes. In contrast to most chemokine receptor genes, the STRL22 coding sequence is not limited to a single exon.

摘要

利用基于趋化因子受体保守序列的引物池进行聚合酶链反应（PCR），我们克隆了G蛋白偶联受体基因家族的一个人类成员。这个新基因，我们命名为STRL22，预计编码一种与趋化因子受体相关的受体，包括IL8RA（CXCR1）、IL8RB（CXCR2）和CXCR4/融合素，以及与孤儿受体EBI1和BLR1相关的受体。与在白细胞生物学中的作用一致，STRL22在淋巴细胞和淋巴组织中表达。我们已将STRL22定位到6号染色体的q27区域。STRL22的互补DNA（cDNA）反映了5'-非翻译区的可变（或不完整）mRNA加工，这是在分析其他趋化因子受体基因时发现的一种现象。与大多数趋化因子受体基因不同，STRL22的编码序列并不局限于单个外显子。