Martinez A J, Robacker D C, Garcia J A
Mission Biological Control Center, USDA, TX 78573, USA.
J Econ Entomol. 1997 Feb;90(1):130-4. doi: 10.1093/jee/90.1.130.
Centrifugation pellets obtained from an isolate of Bacillus thuringiensis subspecies darmstadiensis (Guat 1) cultured from a Guatemalan soil sample were found to be toxic to Anastrepha ludens (Loew) adults in the laboratory. We developed a bioassay diet that consisted of a mixture of the bacterium, a protein source, and sugar. A pH of 4.1 of the mixture was needed to obtain maximum adult mortality. One meal of the diet, which lasted from 30 s to 4 min, was enough to cause > 70% mortality of both fed or starved adults. Mortality of fed adults was 70-75% following a feeding period of 60 min and mortality of starved adults was 80-90% following a feeding period of 30 min. The isolate was toxic to adults from 1 to 21 d old.
从危地马拉土壤样本中培养出的苏云金芽孢杆菌达姆斯塔迪亚种（Guat 1）分离株获得的离心沉淀物，在实验室中被发现对墨西哥实蝇成虫有毒。我们开发了一种生物测定饲料，其由细菌、蛋白质来源和糖的混合物组成。混合物的pH值为4.1时可获得最高的成虫死亡率。一餐持续30秒至4分钟的饲料足以导致喂食或饥饿的成虫死亡率超过70%。喂食60分钟后，喂食成虫的死亡率为70 - 75%，喂食30分钟后，饥饿成虫的死亡率为80 - 90%。该分离株对1至21日龄的成虫有毒。
