中风倾向型自发性高血压大鼠培养的系膜细胞中内皮素A受体基因的表达增强。

Augmented expression of the endothelin-A receptor gene in cultured mesangial cells from stroke-prone spontaneously hypertensive rats.

Hiraoka J, Arai H, Yoshimasa T, Takaya K, Miyamoto Y, Yamashita J, Suga S, Ogawa Y, Shirakami G, Itoh H

Graduate School of Human and Environmental Studies, Kyoto University, Japan.

Clin Exp Pharmacol Physiol Suppl. 1995 Dec;22(1):S191-2. doi: 10.1111/j.1440-1681.1995.tb02876.x.

Abstract

To elucidate the role of endothelin (ET) receptor in hypertension, we studied the expression of the ET-A receptor (ET-AR) gene and the ET-B receptor (ET-BR) gene in cultured mesangial cells isolated from stroke-prone spontaneously hypertensive rats (SHRSP) and Wistar-Kyoto (WKY) rats. 2. The mesangial cells from both SHRSP and WKY expressed ET-AR predominantly. The level of the ET-AR mRNA in mesangial cells from SHRSP was 5-fold higher than that in the cells from WKY. The ET-BR mRNA in the mesangial cells from both strains was hardly detectable by northern blot analysis. 3. These results demonstrate that the expression of the ET-AR gene was markedly augmented in mesangial cells from SHRSP.

摘要

为阐明内皮素（ET）受体在高血压中的作用，我们研究了从易卒中型自发性高血压大鼠（SHRSP）和Wistar - Kyoto（WKY）大鼠分离的培养系膜细胞中ET - A受体（ET - AR）基因和ET - B受体（ET - BR）基因的表达。2. SHRSP和WKY的系膜细胞均主要表达ET - AR。SHRSP系膜细胞中ET - AR mRNA水平比WKY细胞中的高5倍。通过Northern印迹分析，两种品系系膜细胞中的ET - BR mRNA几乎检测不到。3. 这些结果表明，SHRSP系膜细胞中ET - AR基因的表达明显增强。