多巴胺诱导成年易卒中型自发性高血压大鼠离体肾内动脉舒张。

Dopamine-induced relaxation in isolated intrarenal arteries from adult stroke-prone spontaneously hypertensive rats.

作者信息

Gao Y J, Nishimura Y, Suzuki A

机构信息

Department of Pharmacology, Kinki University School of Medicine, Osaka-Sayama, Japan.

出版信息

Clin Exp Pharmacol Physiol Suppl. 1995 Dec;22(1):S96-8. doi: 10.1111/j.1440-1681.1995.tb02985.x.

DOI:10.1111/j.1440-1681.1995.tb02985.x

Abstract

The relaxant effects of dopamine (DA) on the intrarenal arteries obtained from 6 month old stroke-prone spontaneously hypertensive rats (SHRSP) and age-matched Wistar-Kyoto (WKY) rats were pharmacologically investigated in vitro. 2. DA (10(-7)-3 x 10(-5) mol/L) produced endothelium-independent relaxation on the arterial rings which had been incubated with phenoxybenzamine (2 x 10(-6) mol/L) and precontracted with KCl. 3. DA-induced relaxation was greater in the arterial rings from SHRSP than in those from WKY. SKF 38393 (10(-8)-10(-6) mol/L) partially mimicked DA-produced relaxation in both groups. SCH 23390 dose-dependently inhibited DA-induced relaxation with pD'2 value of 9.33 for SHRSP and of 9.26 for WKY. 4. There were no significant differences between SHRSP and WKY in the relaxation caused by forskolin, dibutyryl cyclic AMP, or 3-isobutyl-1-methylxanthine. 5. These results suggested that DA1 receptor-mediated relaxation was increased in the intrarenal arteries from SHRSP, and this increase might not be associated with altered vasodilation mediated by cyclic AMP.

摘要

采用体外药理学方法研究了多巴胺（DA）对6月龄易卒中型自发性高血压大鼠（SHRSP）及年龄匹配的Wistar-Kyoto（WKY）大鼠肾内动脉的舒张作用。2. DA（10⁻⁷ - 3×10⁻⁵ mol/L）对预先用酚苄明（2×10⁻⁶ mol/L）孵育并经氯化钾预收缩的动脉环产生非内皮依赖性舒张作用。3. DA诱导的舒张作用在SHRSP的动脉环中比在WKY的动脉环中更强。SKF 38393（10⁻⁸ - 10⁻⁶ mol/L）在两组中部分模拟了DA产生的舒张作用。SCH 23390剂量依赖性地抑制DA诱导的舒张作用，SHRSP的pD'2值为9.33，WKY的pD'2值为9.26。4. 对于福司可林、二丁酰环磷腺苷或3-异丁基-1-甲基黄嘌呤引起的舒张作用，SHRSP和WKY之间没有显著差异。5. 这些结果表明，SHRSP肾内动脉中DA1受体介导的舒张作用增强，且这种增强可能与环磷腺苷介导的血管舒张改变无关。