Fajac I, Roisman G L, Lacronique J, Polla B S, Dusser D J
Unite de Formation et de Recherche, Cochin-Port Royal, Universite Rene Descartes, Paris, France.
Eur Respir J. 1997 Mar;10(3):633-8.
Heat shock proteins (HSPs), which are important targets for gammadelta T-lymphocytes, are thought to play a role in inflammatory and immune diseases. The purpose of this study was to characterize, in asthma, the presence and distribution of alphabeta and gammadelta T-lymphocytes and of hsp60, hsp70 and hsp90 in bronchial biopsies, and to seek for a co-localization of gammadelta T-cells and HSPs. Ten subjects with mild atopic asthma and nine control subjects underwent fibreoptic bronchoscopy and bronchial biopsies, to which specific monoclonal antibodies and immunohistochemical techniques were applied. T-lymphocytes present in bronchi both of asthmatic and control subjects were predominantly of the alphabeta T-cell receptor phenotype (median 642 cells x mm(-2) (range 85-1,510 cells x mm(-2)), and 855 cells x mm(-2) (286-2,424 cells x mm(-2)), respectively), whereas, gammadelta T-lymphocytes were always rare (median 26 cells x mm(-2) (range 0-114 cells x mm(-2)), and 0 cells x mm(-2 (0-57 cells x mm(-2), respectively). Both in asthmatic and control subjects, bronchial epithelium was positive for hsp60, hsp70 and hsp90. There was no significant difference in the percentages of positive epithelial cells between asthmatic and control subjects. No co-localization of HSPs and gammadelta T-cells was observed. Our findings do not support the hypothesis that heat shock proteins and gammadelta T-cells play an important role in inflammatory and immune responses in mild asthma.
热休克蛋白（HSPs）是γδ T淋巴细胞的重要靶点，被认为在炎症和免疫疾病中发挥作用。本研究的目的是在哮喘患者的支气管活检中，对αβ和γδ T淋巴细胞以及hsp60、hsp70和hsp90的存在和分布进行表征，并寻找γδ T细胞与热休克蛋白的共定位。10名轻度特应性哮喘患者和9名对照受试者接受了纤维支气管镜检查和支气管活检，并应用了特异性单克隆抗体和免疫组织化学技术。哮喘患者和对照受试者支气管中的T淋巴细胞主要为αβ T细胞受体表型（中位数分别为642个细胞×mm⁻²（范围85 - 1510个细胞×mm⁻²）和855个细胞×mm⁻²（286 - 2424个细胞×mm⁻²）），而γδ T淋巴细胞总是很少见（中位数分别为26个细胞×mm⁻²（范围0 - 114个细胞×mm⁻²）和0个细胞×mm⁻²（0 - 57个细胞×mm⁻²））。在哮喘患者和对照受试者中，支气管上皮细胞的hsp60、hsp70和hsp90均呈阳性。哮喘患者和对照受试者之间阳性上皮细胞的百分比无显著差异。未观察到热休克蛋白与γδ T细胞的共定位。我们的研究结果不支持热休克蛋白和γδ T细胞在轻度哮喘的炎症和免疫反应中起重要作用这一假说。
