Gordhan B G, Mizrahi V
Molecular Biology Unit, South African Institute for Medical Research, Johannesburg.
Gene. 1997 Mar 10;187(1):63-6. doi: 10.1016/s0378-1119(96)00707-x.
A polA mutant of Mycobacterium smegmatis (Ms) lacking DNA polymerase activity could not support the replication of a pAL5000-derived plasmid or a derivative harbouring the RLEP-flanked polA gene from M. leprae (Mlep). In contrast, the plasmid containing the M. tuberculosis polA gene transformed the mutant with high efficiency and complemented its damage-sensitive phenotype suggesting that the replication of the pAL5000 origin is dependent on host PolI function and that the RLEP-flanked Mlep polA gene is not expressed in Ms.
