Huberts P, Mizrahi V
Department of Hematology, University of the Witwatersrand Medical School, Johannesburg, South Africa.
Gene. 1995 Oct 16;164(1):133-6. doi: 10.1016/0378-1119(95)00453-d.
The polA gene (encoding DNA polymerase I) from Mycobacterium tuberculosis was cloned using an internal gene segment probe generated by PCR amplification of genomic DNA [Mizrahi et al., Gene 136 (1993) 287-290]. The gene encodes a polypeptide 904 amino acids (aa) in length that shares 89% identity with a 911-aa homologue from Mycobacterium leprae. The polypeptide has all of the primary structural elements necessary for DNA polymerase and 5'-3' exonuclease activity, but lacks the motifs required for an associated 3'-5' exonuclease (proofreading) activity.
利用通过对基因组DNA进行PCR扩增产生的内部基因片段探针，克隆了结核分枝杆菌的polA基因（编码DNA聚合酶I）[米兹拉希等人，《基因》136（1993）287 - 290]。该基因编码一个长度为904个氨基酸（aa）的多肽，与麻风分枝杆菌的一个911个氨基酸的同源物具有89%的同一性。该多肽具有DNA聚合酶和5'-3'核酸外切酶活性所需的所有主要结构元件，但缺乏相关3'-5'核酸外切酶（校对）活性所需的基序。
