Mohanty K C, Naik D R
Department of Zoology, Ravenshaw College, Cuttack, India.
Gen Comp Endocrinol. 1997 Mar;105(3):302-13. doi: 10.1006/gcen.1996.6831.
Immunocharacteristics of the adenohypophysial cells of the rat snake Ptyas mucosus (Colubridae) were studied with an unlabeled antibody enzyme technique (PAP method) using rabbit antisera against mammalian/synthetic hypophysial hormones. Adenohypophysial cells were identified on the basis of their specific immunoreactivity with various heterologous antisera. As in other reptiles, there was regional localization of pars distalis (PD) cell types in this snake. The gonadotropic (GTH) cells were identified by their specific immunoreactivity with anti-porcine (p)LH beta serum and were distributed uniformly throughout the PD. The thyrotropic (TSH) cells, recognized by their specific immunoreactivity with anti-human (h)TSH beta serum, were found in the medial PD. The prolactin (PRL) and growth hormone (GH) cells were revealed by their specific immunoreactivity with anti-ovine (o)PRL and anti-hGH sera, respectively; the former were confined to the anterior two-thirds of the PD, and the latter were restricted to the posterior third of the PD. The corticotropic (ACTH) cells, identified by their specific immunoreactivity with the anti-ACTH1-24 serum, were localized in the anterior two-thirds of the PD. Though both ACTH and PRL cells were confined to the same area of the PD, they could be distinguished by their distinctive morphology and distribution pattern. Cells of the pars intermedia were revealed by their immunoreactivity to anti-alpha MSH (melanophore-stimulating hormone) and anti-ACTH1-24 sera. Among each adenohypophysial cell type, there was variation in the intensity of immunoreactivity and morphological features, which may be due to their heterogeneity, reflecting various stages of cellular activity. Unlike most other snake species, the occurrence of a rudimentary pars tuberalis in P. mucosus containing a few immunoreactive GTH and TSH cells appeared to be a novel finding. The occurrence of the PRL-like and TSH-like immunoreactivity seen in certain neuronal perikarya and fibers of the hypothalamus and median eminence conforms with earlier observations in other tetrapods. To resolve certain discrepancies in the literature, the tinctorial affinities of immunohistochemically identified adenohypophysial cell types of P. mucosus were studied using various conventional staining methods and were compared with those of other reptilian species studied earlier, including snakes of the family Colubridae.
采用未标记抗体酶技术（PAP法），使用针对哺乳动物/合成垂体激素的兔抗血清，研究了鼠蛇（游蛇科）腺垂体细胞的免疫特性。根据腺垂体细胞与各种异源抗血清的特异性免疫反应来鉴定它们。与其他爬行动物一样，这种蛇的远侧部（PD）细胞类型存在区域定位。促性腺激素（GTH）细胞通过与抗猪（p）LHβ血清的特异性免疫反应来鉴定，它们均匀分布于整个PD。促甲状腺激素（TSH）细胞通过与抗人（h）TSHβ血清的特异性免疫反应来识别，位于PD的内侧。催乳素（PRL）细胞和生长激素（GH）细胞分别通过与抗羊（o）PRL和抗hGH血清的特异性免疫反应而显示出来；前者局限于PD的前三分之二，后者局限于PD的后三分之一。促肾上腺皮质激素（ACTH）细胞通过与抗ACTH1 - 24血清的特异性免疫反应来鉴定，位于PD的前三分之二。虽然ACTH细胞和PRL细胞都局限于PD的同一区域，但它们可以通过独特的形态和分布模式来区分。中间部细胞通过它们对抗α - MSH（促黑素细胞激素）和抗ACTH1 - 24血清的免疫反应而显示出来。在每种腺垂体细胞类型中，免疫反应强度和形态特征存在差异，这可能是由于它们的异质性，反映了细胞活动的不同阶段。与大多数其他蛇类不同，鼠蛇中出现含有少量免疫反应性GTH和TSH细胞的原始结节部似乎是一个新发现。在下丘脑和正中隆起的某些神经元胞体和纤维中观察到的PRL样和TSH样免疫反应与其他四足动物早期的观察结果一致。为了解决文献中的某些差异，使用各种传统染色方法研究了鼠蛇免疫组织化学鉴定的腺垂体细胞类型的染色亲和力，并与早期研究的其他爬行动物物种（包括游蛇科的蛇）进行了比较。
