Kasper Romano Silvio, Shved Natallia, Takahashi Akiyoshi, Reinecke Manfred, Eppler Elisabeth
Division of Neuroendocrinology, Institute of Anatomy, University of Zürich, Winterthurerstrasse 190, Zürich, CH-8057, Switzerland.
Cell Tissue Res. 2006 Aug;325(2):303-13. doi: 10.1007/s00441-005-0119-7. Epub 2006 Mar 22.
Fish pituitary plays a central role in the control of growth, development, reproduction and adaptation to the environment. Several types of hormone-secreting adenohypophyseal cells have been characterised and localised in diverse teleost species. The results suggest a similar distribution pattern among the species investigated. However, most studies deal with a single hormone or hormone family. Thus, we studied adjacent sections of the pituitary of Oreochromis niloticus, the tilapia, by conventional staining and immunohistochemistry with specific antisera directed against growth hormone (GH), prolactin (PRL), somatolactin (SL), thyrotropin (beta-TSH), follicle-stimulating hormone (beta-FSH), luteinising hormone (beta-LH), adrenocorticotropic hormone (ACTH) and melanocyte-stimulating hormone (alpha-MSH). The pituitary was characterised by a close interdigitating neighbourhood of neurohypophysis (PN) and adenohypophysis. PRL-immunoreactive and ACTH-immunoreactive cells were detected in the rostral pars distalis. GH-immunoreactive cells were present in the proximal pars distalis (PPD). A small region of the PPD contained beta-TSH-immunoreactive cells, and beta-LH-immunoreactive cells covered approximately the remaining parts. Centrally, beta-FSH-immunoreactive cells were detected in the vicinity of the GH-containing cells. Some of these cells also displayed beta-LH immunoreactivity. The pars intermedia was characterised by branches of the PN surrounded by SL-containing and alpha-MSH-immunoreactive cells. The ACTH and alpha-MSH antisera were observed to cross-react with the respective antigens. This cross-reactivity was abolished by pre-absorption. We present a complete map of the distinct localisation sites for the classical pituitary hormones, thereby providing a solid basis for future research on teleost pituitary.
鱼类垂体在生长、发育、繁殖以及对环境的适应控制中起着核心作用。在多种硬骨鱼物种中，已对几种分泌激素的腺垂体细胞进行了特征描述和定位。结果表明在所研究的物种中存在相似的分布模式。然而，大多数研究仅涉及单一激素或激素家族。因此，我们通过常规染色和免疫组织化学方法，使用针对生长激素（GH）、催乳素（PRL）、生长抑素（SL）、促甲状腺激素（β-TSH）、促卵泡激素（β-FSH）、黄体生成素（β-LH）、促肾上腺皮质激素（ACTH）和促黑素细胞激素（α-MSH）的特异性抗血清，对尼罗罗非鱼（罗非鱼）垂体的相邻切片进行了研究。垂体的特征是神经垂体（PN）和腺垂体紧密交错相邻。在远侧部前端检测到PRL免疫反应性细胞和ACTH免疫反应性细胞。GH免疫反应性细胞存在于远侧部近端（PPD）。PPD的一小区域含有β-TSH免疫反应性细胞，β-LH免疫反应性细胞覆盖了大约其余部分。在中央，在含GH细胞附近检测到β-FSH免疫反应性细胞。其中一些细胞也显示出β-LH免疫反应性。中间部的特征是PN的分支被含SL和α-MSH免疫反应性细胞包围。观察到ACTH和α-MSH抗血清与各自的抗原发生交叉反应。这种交叉反应通过预吸收被消除。我们展示了经典垂体激素不同定位位点的完整图谱，从而为未来硬骨鱼垂体研究提供了坚实的基础。
