Goldwurm S, Menzies M L, Banyer J L, Powell L W, Jazwinska E C
The Queensland Institute of Medical Research, University of Queensland, Brisbane, Queensland, 4029, Australia.
Genomics. 1997 Mar 15;40(3):486-9. doi: 10.1006/geno.1996.4583.
cDNA selection and exon trapping were performed on cosmids mapping to a region 3 Mb distal to HLA-A. Analysis of resulting fragments indicated the presence of two zinc finger transcripts, and one of these was used to isolate a partial cDNA (ZNF184) from a placental library. The second transcript contained additional sequence of the 5' end of the gene, extending the sequence to 2678 bp. Sequence analysis indicates that ZNF184 is a classical Krueppel zinc finger with 19 highly conserved zinc finger motifs at the C-terminus and a Krueppel associated box at the N-terminus of the protein. This gene encodes a 3.2-kb transcript that is highly expressed in testis and expressed at a moderate to low level in all other tissues tested. This zinc finger gene maps to a region approximately 200 kb distal to the microsatellite marker D6S105 and approximately 300 kb proximal to D6S1260.
对定位于HLA - A远端3 Mb区域的黏粒进行了cDNA筛选和外显子捕获。对所得片段的分析表明存在两种锌指转录本，其中之一被用于从胎盘文库中分离出一个部分cDNA（ZNF184）。第二个转录本包含该基因5'端的额外序列，使序列延伸至2678 bp。序列分析表明，ZNF184是一个经典的克鲁ppel锌指，在蛋白质的C端有19个高度保守的锌指基序，在N端有一个克鲁ppel相关框。该基因编码一个3.2 kb的转录本，在睾丸中高度表达，在所有其他测试组织中表达水平为中度至低度。这个锌指基因定位于微卫星标记D6S105远端约200 kb处，D6S1260近端约300 kb处。
