肠球菌信息素响应性细胞溶素质粒pAD1的转移起始点（oriT）位于repA决定簇内。
The origin of transfer (oriT) of the enterococcal, pheromone-responding, cytolysin plasmid pAD1 is located within the repA determinant.
作者信息
An F Y, Clewell D B
机构信息
School of Dentistry, The University of Michigan, Ann Arbor, Michigan 48109, USA.
出版信息
Plasmid. 1997;37(1):87-94. doi: 10.1006/plas.1996.1270.
PMID:9073585
Abstract
The highly conjugative pAD1 plasmid encodes a mating response to the peptide sex pheromone cAD1 secreted by recipient strains of Enterococcus faecalis. The location of the origin of transfer (oriT) was determined through the ability to mobilize the Escherichia coli-E. faecalis shuttle plasmid pAM401 carrying specific segments of pAD1. The oriT sequence was found to be completely within the repA determinant known to be required for plasmid maintenance.
摘要
高接合性的pAD1质粒编码对粪肠球菌受体菌株分泌的肽性信息素cAD1的交配反应。通过动员携带pAD1特定片段的大肠杆菌-粪肠球菌穿梭质粒pAM401的能力来确定转移起始点（oriT）的位置。发现oriT序列完全位于已知质粒维持所需的repA决定簇内。
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