Wagner J E, Yamanaka W, Typpo J T, Kusewitt D F
Arch Latinoam Nutr. 1977 Mar;27(1):17-31.
Feeding a protein deficient diet containing corn and beans as the protein source for 100 days produced marked malnutrition in guinea pigs. The organs most affected were the liver, pancreas, and testis. Some of the clinical and histological manifestations included growth retardation, atrophy of the liver, decreased number and size of islets of Langerhans, and sterility in male animals. The animals showed clinical signs of protein-calorie malnutrition. This could be the consequence of malabsorption, poor nutrient utilization, and reduced food intake which led to inadequate dietary protein and, secondarily, to inadequate levels of other essential nutrients, especially calories. The low protein level and low biological value of plant proteins used in this study were especially effective in producing the severe pathological changes associated with chronic protein-calorie malnutrition. Some of the changes were marked and incompatible with normal function.
以玉米和豆类作为蛋白质来源，给豚鼠喂食缺乏蛋白质的饮食100天，导致豚鼠出现明显的营养不良。受影响最严重的器官是肝脏、胰腺和睾丸。一些临床和组织学表现包括生长迟缓、肝脏萎缩、胰岛数量和大小减少以及雄性动物不育。这些动物表现出蛋白质 - 热量营养不良的临床症状。这可能是吸收不良、营养利用不佳和食物摄入量减少的结果，这些因素导致膳食蛋白质不足，进而导致其他必需营养素水平不足，尤其是热量不足。本研究中使用的植物蛋白的低蛋白水平和低生物学价值在产生与慢性蛋白质 - 热量营养不良相关的严重病理变化方面特别有效。其中一些变化很明显，与正常功能不相容。
