Adult male Sprague-Dawley rats were subjected to the fear-potentiated startle, elevated plus-maze and social interaction tests and the levels of norepinephrine, dopamine and serotonin in the dorsomedial hypothalamus were determined by high-performance liquid chromatography. 2. Only rats subjected to the full fear potentiated startle test and not the other tests of anxiety or components (foot shocks or acoustic startle) of fear-potentiated startle showed significant increases in norepinephrine and dopamine levels after 24 hours. 3. A time course experiment specific for the norepinephrine changes in the dorsomedial hypothalamus of fear-potentiated startle rats revealed a significant increase in tissue content as compared to controls at both the 12 and 24 hour post-test time points. 4. Tyrosine hydroxylase activity in the dorsomedial hypothalamus of the fear-potentiated startle rats did not show a significant change from controls. 5. An in vitro release study found a significant decrease in potassium-stimulated release of norepinephrine in the dorsomedial hypothalamus as compared to controls at 24 hours post-test. 6. These results suggest that animals exposed to fear-potentiated startle and not other tests of "anxiety" have a change in tissue catecholamine levels and that the norepinephrine change may be the result of a decrease in release and not an increase in synthesis.
