在稀羟乙基纤维素溶液中对超螺旋和线性DNA进行毛细管电泳。

Capillary electrophoresis of supercoiled and linear DNA in dilute hydroxyethyl cellulose solution.

作者信息

Hammond R W, Oana H, Schwinefus J J, Bonadio J, Levy R J, Morris M D

机构信息

Department of Chemistry, School of Medicine, University of Michigan, Ann Arbor 48109, USA.

出版信息

Anal Chem. 1997 Mar 15;69(6):1192-6. doi: 10.1021/ac960980z.

DOI:10.1021/ac960980z

PMID:9075407

Abstract

Capillary electrophoresis in dilute hydroxyethyl cellulose is shown to separate supercoiled DNA in the size range 2000-16,000 base pairs. The plasmids migrate more slowly than linear ds-DNA of the same sizes. Plasmid bandwidths are larger than observed for ds-DNA, allowing identification of the type of DNA by bandwidth. The differing dependence of mobility on chain length can be explained by assuming that a plasmid migrates as an elastic rod, while ds-DNA migrates as a wormlike chain.

摘要

稀羟乙基纤维素中的毛细管电泳可分离大小在2000 - 16,000碱基对范围内的超螺旋DNA。这些质粒的迁移速度比相同大小的线性双链DNA慢。质粒的带宽比双链DNA的带宽大，可通过带宽识别DNA的类型。迁移率对链长的不同依赖性可以通过假设质粒以弹性杆的形式迁移，而双链DNA以蠕虫状链的形式迁移来解释。