de Carmejane O, Schwinefus J J, Wang S C, Morris M D
Department of Chemistry, University of Michigan, Ann Arbor 48109-1055, USA.
J Chromatogr A. 1999 Jul 16;849(1):267-76. doi: 10.1016/s0021-9673(99)00527-0.
We report electrophoretic separation of supercoiled plasmids (2-16 kilo base pairs) and linear double-stranded DNA (0.6-23 kilo base pairs) in uncoated capillaries filled with dilute hydroxyethylcellulose. Because electroosmotic flow reverses the order of elution, long plasmids spend less time in the capillary and their bandwidths are narrower than observed in coated capillaries. However, resolution is similar to that obtained in coated capillaries, because it is governed by the distribution of unresolved topoisomers. In the presence of electroosmotic flow migration of supercoiled plasmids does not follow the elastic rod model that has been observed in coated capillaries.
我们报道了在填充有稀羟乙基纤维素的未涂层毛细管中对超螺旋质粒（2 - 16千碱基对）和线性双链DNA（0.6 - 23千碱基对）进行的电泳分离。由于电渗流会使洗脱顺序颠倒，长质粒在毛细管中停留的时间较短，并且它们的带宽比在涂层毛细管中观察到的要窄。然而，分辨率与在涂层毛细管中获得的分辨率相似，因为它由未分离的拓扑异构体的分布决定。在电渗流存在的情况下，超螺旋质粒的迁移并不遵循在涂层毛细管中观察到的弹性杆模型。
