Hypotension during hemodialysis: role for nitric oxide.

Hypotensive episodes during hemodialysis are a frequent complication in patients with end-stage renal disease. The possibility that nitric oxide (NO), a major regulator of cardiovascular hemodynamics, could be a factor was explored. Pre and postdialysis plasma samples from 17 hemodialysis patients were analyzed for the stable end products of NO,nitrite + nitrate (NO2 + NO3), by the Greiss method. Predialysis NO2 + NO3 levels were significantly higher in end-stage renal disease than in nine age-matched controls (44.08 +/- standard error of mean 5.74 versus 18.67 +/- 3.56 uM, P = 0.017). In more than half of the patients, postdialysis values dropped markedly, whereas in others the value change was far less; several rose above predialysis values. Depending on the nitrite + nitrate reduction ratio (pre minus postdialysis NO2 + NO3 divided by the predialysis value) patients were separated into two groups, A (n = 9 where nitrate + nitrate reduction ratio was > 0.5 and B (n = 8 where nitrate + nitrate reduction ratio was < 0.5). Whereas the mean predialysis NO2 + NO3 values between groups A and B did not differ significantly, postdialysis levels fell from a predialysis mean of 50 uM to 12 uM in group A but rose from 37 uM to 45 uM in group B. The difference between the postdialysis values of group A and group B was significant (P = 0.0264). In group B, mean systolic blood pressure dropped more than in group A, (57.8 mm Hg compared with 21.2 mm Hg, P = 0.0078). When measured by analysis of variance for repeated measures, skin and core temperatures and blood pressures were lower in group B than in group A. The volume of the ultrafiltrate was removed and dialysis duration and mean weight loss did not differ. Thus, in group B, apparently NO formation increased during hemodialysis exceeding the rate of removal or metabolism of the end products, whereas in group A, NO2 + NO3 removal or metabolism was without apparent increase in the formation of NO. The basis for this difference is unknown. Because vasodilation is a major effect of NO, the strong association of severe reduction in blood pressures and increased NO synthesis in subset B suggests a role for NO in hypotensive episodes during hemodialysis.