Rigby M A, Hosie M J, Willett B J, Mackay N, McDonald M, Cannon C, Dunsford T, Jarrett O, Neil J C
Department of Veterinary Pathology, University of Glasgow Veterinary School, UK.
AIDS Res Hum Retroviruses. 1997 Mar 20;13(5):405-12. doi: 10.1089/aid.1997.13.405.
Direct inoculation of genetic material in DNA form is a novel approach to vaccination that has proved efficacious for a number of viral agents. We are interested in the potential of this approach for the delivery of vaccines based on attenuated or replication-defective retroviruses. Toward this goal, we tested the effect of intramuscular inoculation of a plasmid containing the entire genome of feline immunodeficiency virus (FIV-Petaluma, F14 clone). DNA delivery was compared with intramuscular or intraperitoneal inoculation of virus reconstituted from the same molecular clone. The outcome was monitored by serological analysis and quantitative virus load determination over a 31-week period. DNA inoculation was found to be a reliable means of infection, although seroconversion and the rise in PBMC virus load were delayed relative to intramuscular or intraperitoneal inoculation of virus. At 31 weeks, similar levels of proviral DNA were detected in central lymphoid tissue of all infected animals. In conclusion, DNA inoculation of proviral DNA will be of use as a novel method of cell-free virus challenge and may have further potential for the delivery of lentiviral vaccines.
直接接种DNA形式的遗传物质是一种新型疫苗接种方法，已被证明对多种病毒制剂有效。我们对这种方法在递送基于减毒或复制缺陷型逆转录病毒的疫苗方面的潜力感兴趣。为了实现这一目标，我们测试了肌肉注射含有猫免疫缺陷病毒（FIV-Petaluma，F14克隆）全基因组的质粒的效果。将DNA递送与从同一分子克隆重建的病毒的肌肉注射或腹腔注射进行比较。在31周的时间内，通过血清学分析和定量病毒载量测定来监测结果。发现DNA接种是一种可靠的感染方式，尽管相对于病毒的肌肉注射或腹腔注射，血清转化和PBMC病毒载量的升高有所延迟。在31周时，在所有感染动物的中枢淋巴组织中检测到相似水平的前病毒DNA。总之，接种前病毒DNA将作为一种新型的无细胞病毒攻击方法，并可能在递送慢病毒疫苗方面具有进一步的潜力。
