de Oca J, Bettonica C, Cuadrado S, Vallet J, Martín E, Garcia A, Montañés T, Jaurrieta E
Department of Surgery, University of Barcelona, Spain.
Transplantation. 1997 Mar 15;63(5):636-9. doi: 10.1097/00007890-199703150-00004.
The aim of this study was to analyze the possible protective effects of a glutamine and arginine precursor (ornithine-alpha-ketoglutarate [OKG]) on the mucosa of a transplanted intestine when administered with either a defined formula oral diet (DFD) or a standard chow. Isogenic male Lewis rats (250 g) were submitted to a laparotomy (groups 1 and 2) or to an orthotopic small bowel transplantation (SBT; groups 3-6). Groups 1, 3, and 5 received a DFD 14 days after surgery. Groups 2, 4, and 6 received standard chow. In addition, groups 5 and 6 received a daily oral supplementation of 1.4 g/kg of OKG. Weight changes and food intake were recorded daily. At the end of the study, bacterial translocation (BT) was measured in mesenteric lymph nodes, liver, and spleen. The protein/DNA index was also determined in intestinal mucosa. SBT induced BT in all transplanted groups, especially in those fed DFD. Addition of OKG (groups 5 and 6) significantly reduced BT in comparison with groups 3 and 4 and improved the protein/DNA index as well as weight gain. It is concluded that OKG supplementation protects the intestinal barrier after SBT, and that this effect is more marked when it is added to a standard chow.
本研究的目的是分析谷氨酰胺和精氨酸前体（鸟氨酸-α-酮戊二酸[OKG]）在与特定配方口服饮食（DFD）或标准饲料一起给予时，对移植肠道黏膜可能产生的保护作用。将同基因雄性Lewis大鼠（250克）进行剖腹手术（第1组和第2组）或原位小肠移植（SBT；第3 - 6组）。第1、3和5组在术后14天接受DFD。第2、4和6组接受标准饲料。此外，第5和6组每天口服补充1.4克/千克的OKG。每天记录体重变化和食物摄入量。在研究结束时，测量肠系膜淋巴结、肝脏和脾脏中的细菌移位（BT）。还测定了肠黏膜中的蛋白质/DNA指数。SBT在所有移植组中均诱导了BT，尤其是在喂食DFD的组中。与第3和4组相比，添加OKG（第5和6组）显著降低了BT，并改善了蛋白质/DNA指数以及体重增加。得出的结论是，补充OKG可保护SBT后的肠道屏障，并且当将其添加到标准饲料中时，这种效果更为明显。
